Plasmid

Part:BBa_J23076:Design

Designed by: John Anderson   Group: iGEM06_Berkeley   (2006-09-06)


pSB3C6-like RFP reporter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 16
    Illegal SpeI site found at 78
    Illegal PstI site found at 961
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal NheI site found at 1808
    Illegal SpeI site found at 78
    Illegal PstI site found at 961
    Illegal NotI site found at 7
    Illegal NotI site found at 954
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 16
    Illegal SpeI site found at 78
    Illegal PstI site found at 961
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 16
    Illegal SpeI site found at 78
    Illegal PstI site found at 961
    Illegal AgeI site found at 657
    Illegal AgeI site found at 769
    Illegal AgeI site found at 1398
    Illegal AgeI site found at 1722
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

N/A


Source

JCA082006-Construction of pSB3C6-Bca1020/r0040=J23076 Digest pBca1020-r0040 . . . . . (EcoRI/PstI, 966 bp, small) Sub into pAC899 . . . . . . . . (EcoRI/PstI) Product is pJ23076, temp is pSB3C6-Bca1020

References